Solid-phase synthesis adds one residue at a time, and each coupling is efficient but imperfect. Those small failures compound, so a long sequence starts from a cruder mixture than a short one before purification begins.
A fifteen-residue peptide can therefore reach a higher purity more readily than an eighty-residue one, with identical care applied to both.
Purification separates by physical behaviour. An impurity that behaves almost identically to the target — a deletion sequence missing one small residue, or an oxidised form — is correspondingly hard to resolve.
This is sequence-dependent. Two peptides of the same length can be quite different to purify depending on what the failure products look like.
As a statement about what that compound by that route can achieve. A supplier quoting an unusually high figure for a difficult molecule is making a claim worth checking against the actual chromatogram.
The informative certificate is the one reporting the method, the measured value for that lot, and the water and residual solvent alongside — not the one with the largest number on it.
Solid-phase synthesis adds one residue at a time, and each coupling is efficient but imperfect. Those small failures compound, so a long sequence starts from a cruder mixture than a short one before purification begins.
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