The identity test that reads a protein one fragment at a time

Published 26 October 2026 · REVIVE LAB UAE Research Desk · 5 min read
Trypsin cleaves a protein after each lysine and arginine. Somatropin has twenty such sites, so digestion yields about twenty peptides of known sequence. Separated by reversed-phase chromatography and detected by ultraviolet absorbance and mass spectrometry, they give a pattern — the map — that is compared with the map of a reference. A matching map confirms the full sequence; a shifted or missing peak locates a substitution, oxidation, deamidation or clip to within one fragment. Run without reducing the disulfides, the map also shows which cysteines are paired.

Why fragment

The intact protein gives one mass and one chromatographic peak, which confirm size but cannot say where a change lies. Fragments are small enough that each has a precise mass and a distinct retention time, so the sequence is read in pieces. A one-dalton deamidation, invisible on the intact protein with most instruments, is clear on a two-kilodalton fragment.

This is why pharmacopoeial identity for proteins rests on the map rather than on intact mass alone.

How the map is read

Each peak is assigned to a fragment by its mass. The reference map is overlaid; peaks that match in position and area are confirmed, and any difference is investigated by fragmenting the aberrant peptide further in the mass spectrometer to find the exact residue. Oxidised methionine, deamidated asparagine and the extra methionine of somatrem each have characteristic signatures.

A map run without reduction keeps disulfide-linked pairs together, so the two bonds of somatropin appear as two joined fragments.

On a certificate

Reported as conforming to the reference map, often with the chromatogram attached. For a research lot it is the strongest identity evidence available.

Somatropin is supplied by REVIVE LAB UAE for laboratory research use only, with identity and purity documented for the batch. It is not for human or veterinary use, and no dosing, administration or protocol guidance is provided.

What it costs

FormatStrengthPriceAvailability
HGH Somatropin Pack24 mg (72 IU)AED 850In stock

Delivery is AED 40 flat and free on orders over AED 700. Payment is cash on delivery anywhere in the UAE, or USDT (TRC20) in advance, which takes 5% off the order total.

Frequently asked

Why fragment?

The intact protein gives one mass and one chromatographic peak, which confirm size but cannot say where a change lies. Fragments are small enough that each has a precise mass and a distinct retention time, so the sequence is read in pieces. A one-dalton deamidation, invisible on the intact protein with most instruments, is clear on a two-kilodalton fragment.

Does REVIVE LAB UAE provide dosing guidance?

No. REVIVE LAB UAE supplies research materials and provides no dosing, administration or protocol guidance of any kind. Every product is sold for laboratory research use only and is not for human or veterinary consumption.

How fast is delivery in the UAE?

Stock is held in Dubai. An order confirmed before 4 PM Dubai time is dispatched the same working day, reaching Dubai and Abu Dhabi the same day and the remaining emirates within 24 hours. Delivery is AED 40 flat and free over AED 700.

HGH Somatropin Pack — in stock, dispatched from Dubai
HPLC-verified, lot certificate on every carton, 24-hour delivery nationwide. Browse the catalogue → All peptides UAE

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Supplied for laboratory research use only. Not for human or veterinary consumption. REVIVE LAB UAE provides no dosing, administration or protocol guidance.