How purity of somatropin is measured, and what the method resolves

Published 24 October 2026 · REVIVE LAB UAE Research Desk · 5 min read
The pharmacopoeial purity method for somatropin is reversed-phase chromatography on a wide-pore column at elevated temperature with a shallow organic gradient. Under these conditions the native protein separates from its oxidised and deamidated variants, from clipped forms and from the extra-methionine form, and the sum of those related proteins is reported against the main peak. The method is validated for the protein and is not interchangeable with a peptide method.

Why proteins need different conditions

A 22 kDa protein diffuses slowly into narrow pores and can partly unfold on a hydrophobic surface, giving broad or split peaks. Wide-pore silica, a column temperature well above ambient and a slow gradient sharpen the peak and make the variants resolvable. The conditions are part of the method and are stated on the certificate.

A peptide method run on a protein gives an uninterpretable result.

What the profile shows

Oxidised forms elute slightly earlier than native; deamidated forms are better resolved by ion-exchange but appear as shoulders here; clipped or truncated forms and the 192-residue form resolve as separate peaks. Each is identified by mass spectrometry once, then recognised by retention time in routine runs.

The main-peak percentage is what is reported as purity.

Together with size exclusion

Reversed phase denatures and dissociates aggregates, so it does not count them; size exclusion does not resolve chemical variants. The two together describe the lot. A certificate carrying only one has described half of it.

Somatropin is supplied by REVIVE LAB UAE for laboratory research use only, with identity and purity documented for the batch. It is not for human or veterinary use, and no dosing, administration or protocol guidance is provided.

What it costs

FormatStrengthPriceAvailability
HGH Somatropin Pack24 mg (72 IU)AED 850In stock

Delivery is AED 40 flat and free on orders over AED 700. Payment is cash on delivery anywhere in the UAE, or USDT (TRC20) in advance, which takes 5% off the order total.

Frequently asked

Why proteins need different conditions?

A 22 kDa protein diffuses slowly into narrow pores and can partly unfold on a hydrophobic surface, giving broad or split peaks. Wide-pore silica, a column temperature well above ambient and a slow gradient sharpen the peak and make the variants resolvable. The conditions are part of the method and are stated on the certificate.

Does REVIVE LAB UAE provide dosing guidance?

No. REVIVE LAB UAE supplies research materials and provides no dosing, administration or protocol guidance of any kind. Every product is sold for laboratory research use only and is not for human or veterinary consumption.

How fast is delivery in the UAE?

Stock is held in Dubai. An order confirmed before 4 PM Dubai time is dispatched the same working day, reaching Dubai and Abu Dhabi the same day and the remaining emirates within 24 hours. Delivery is AED 40 flat and free over AED 700.

HGH Somatropin Pack — in stock, dispatched from Dubai
HPLC-verified, lot certificate on every carton, 24-hour delivery nationwide. Browse the catalogue → All peptides UAE

Related reading

Supplied for laboratory research use only. Not for human or veterinary consumption. REVIVE LAB UAE provides no dosing, administration or protocol guidance.