The method that counts aggregates in a somatropin lot

Published 24 October 2026 · REVIVE LAB UAE Research Desk · 5 min read
Size-exclusion chromatography passes the sample through a porous column in which small molecules enter the pores and are delayed while large ones pass around them and elute first. Somatropin dimers and larger aggregates therefore elute before the monomer, and the fraction of the total signal in those early peaks is the aggregate content. Pharmacopoeial monographs set a limit on dimer and related substances of higher molecular mass, and the method is non-denaturing so it reports the species present in solution.

How the separation works

No binding is involved. The column is packed with beads of defined pore size in a buffer that keeps the protein native. Elution volume is a function of hydrodynamic size alone, so a dimer at 44 kDa separates cleanly from a 22 kDa monomer and larger aggregates elute at the void.

Detection is by ultraviolet absorbance; the peak areas give the proportions.

Why aggregates are reported

Aggregates are the species most associated with immunogenicity for therapeutic proteins, and they form through the handling stresses somatropin is prone to — interfaces, agitation, freeze-thaw. The figure therefore reports both the manufacturing quality and the handling history of a lot.

Covalent dimers, through disulfide scrambling, and non-covalent ones both appear.

On a certificate

Reported as per cent monomer, or per cent dimer and higher, under the stated column and buffer. It is a line a peptide certificate does not carry and a protein certificate should.

Somatropin is supplied by REVIVE LAB UAE for laboratory research use only, with identity and purity documented for the batch. It is not for human or veterinary use, and no dosing, administration or protocol guidance is provided.

What it costs

FormatStrengthPriceAvailability
HGH Somatropin Pack24 mg (72 IU)AED 850In stock

Delivery is AED 40 flat and free on orders over AED 700. Payment is cash on delivery anywhere in the UAE, or USDT (TRC20) in advance, which takes 5% off the order total.

Frequently asked

How the separation works?

No binding is involved. The column is packed with beads of defined pore size in a buffer that keeps the protein native. Elution volume is a function of hydrodynamic size alone, so a dimer at 44 kDa separates cleanly from a 22 kDa monomer and larger aggregates elute at the void.

Does REVIVE LAB UAE provide dosing guidance?

No. REVIVE LAB UAE supplies research materials and provides no dosing, administration or protocol guidance of any kind. Every product is sold for laboratory research use only and is not for human or veterinary consumption.

How fast is delivery in the UAE?

Stock is held in Dubai. An order confirmed before 4 PM Dubai time is dispatched the same working day, reaching Dubai and Abu Dhabi the same day and the remaining emirates within 24 hours. Delivery is AED 40 flat and free over AED 700.

HGH Somatropin Pack — in stock, dispatched from Dubai
HPLC-verified, lot certificate on every carton, 24-hour delivery nationwide. Browse the catalogue → All peptides UAE

Related reading

Supplied for laboratory research use only. Not for human or veterinary consumption. REVIVE LAB UAE provides no dosing, administration or protocol guidance.